High-temperature sterilizer use method - Database & Sql Blog Articles

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. First, remove the inner layer sterilization barrel, and then add an appropriate amount of water to the outer layer pot to make the water surface and the triangle shelf level.

2. Put back the sterilization bucket and load the items to be sterilized. Be careful not to pack too much, so as not to hinder the circulation of steam and affect the sterilization effect. Do not contact the barrel end of the flask and the end of the tube to prevent the condensate from drenching the paper at the mouth and penetrating into the tampon.

3. Cap and insert the exhaust hose of the cover into the exhaust groove of the inner sterilization tank. Then tighten the two bolts at the same time in a symmetrical manner to make the bolts tight and not leaking.

4. Heat with an electric stove or gas and open the exhaust valve at the same time to boil the water to remove the cold air from the pot. After the cold air is completely drained, close the exhaust valve so that the temperature inside the pot gradually increases as the steam pressure increases. When the pressure in the pot rises to the desired pressure, the heat source is controlled to maintain the pressure for the required time. This experiment was sterilized with 1.05 kg/cm 2 , 121.3 ° C for 20 minutes.

5. After the time required for sterilization, turn off the power or turn off the gas, let the temperature inside the sterilizer drop naturally. When the pressure of the pressure gauge drops to 0, open the vent valve, loosen the bolt, open the lid, and take out the sterilized items. If the pressure does not drop to 0, open the exhaust valve, the pressure in the pot will suddenly drop, so that the medium in the container will be flushed out of the flask mouth or the test tube mouth due to the imbalance of internal and external pressure, causing the cotton plug to contaminate the medium. Pollution.

6. The sterilized medium taken out was placed in a 37 ° C incubator for 24 hours, and it was checked if no bacteria were grown.

However, there are a few points to note:

1. There must be enough water in the pot.

2. The air tightness of the pot must be very good.

3. After use, be sure to put it at the right temperature before you can open it.

Shangyu Star Equipment Co., Ltd.

Located in Daoxu Town, Shangyu District, Shaoxing City, Zhejiang Province, it is an enterprise integrating production, product research and development, processing and trade. The company's main products: drying box series, incubator series, laboratory muffle furnace series, distilled water series, as well as clean workbench, air shower, fume hood, biological safety cabinet and other complete purification equipment.

The company has a strong technical research and development team, a strong after-sales support service system, is the leader in China's laboratory complete sets of equipment manufacturers, welcome customers to buy.