Star Science tells you what is the specific role of the pathogen ELISA test kit? -Huaqiang Electronic Network

ELISA Test Kit Use The ELISA test kit is an anti-human hepatitis E (HEV) virus IgM antibody for qualitative detection of human serum or plasma in vitro. ELISA test kit detection principle ELISA test kit uses qualitative sandwich immunoassay technology to coat microplate plates with synthetic HEV peptide antigen. These peptides are highly antigenic peptides in the core amino acid sequence of Chinese type HEV strain. , from open reading frame 2 and open reading frame 3 of the strain, respectively. Samples or standards are added to the wells and incubated, and if HEV IgM antibodies are present, these antibodies bind to the polypeptide antigen of the HEV and are immobilized thereon, washing the plate to remove other non-specific antibodies and other components in the sample. Then, the goat anti-human IgM-HRP (horseradish peroxidase) enzyme conjugate was added. After the second incubation, the enzyme conjugate was combined with the HEV IgM antibody bound to the first incubation, and the plate was removed. The bound enzyme conjugate is added to the TMB (3,3',5,5'-tetramethylbenzidine) substrate solution, and an enzyme-substrate reaction occurs during the third incubation, only those containing HEV IgM antibodies and The color of the pores of the complex formed by the enzyme conjugate is changed. The reaction between the enzyme and the substrate is terminated by adding a sulfuric acid solution, and the OD value is measured at a wavelength of 450 nm. According to the test standard of the HEV IgM antibody elisa kit, Samples with an OD value greater than or equal to the Cut-Off value are considered to be positive for the initial test. The ELISA kit contains the following components: (1) a dilution of the conjugate and the sample; (2) a washing solution, in a plate ELISA, a commonly used diluent is 0.05% Tween 20 phosphate buffered saline; (3) an enzyme Labeled antigen or antibody (conjugate); (4) substrate for the enzyme; (5) negative control and positive control (in qualitative assay), ELISA kit reference standard and control serum (quantitative determination); 6) Solid phase carrier (immunoadsorbent) which has been coated with antigen or antibody; Star Biosciences distributes many well-known brand ELISA kits in the international life science field, and is one of the authoritative suppliers of scientific research reagents in China. Recognized by the scientific research institutions of the college and designated as the long-term supplier of Elisa kit, as a strategic partner, you are welcome to inquire and order!